Global Phosphoproteomic Mapping of Early Mitotic Exit in Human Cells Identifies Novel Substrate Dephosphorylation Motifs
Identifieur interne : 002D41 ( Main/Exploration ); précédent : 002D40; suivant : 002D42Global Phosphoproteomic Mapping of Early Mitotic Exit in Human Cells Identifies Novel Substrate Dephosphorylation Motifs
Auteurs : Rachael A. Mccloy [Australie] ; Benjamin L. Parker [Australie] ; Samuel Rogers [Australie] ; Rima Chaudhuri [Australie] ; Velimir Gayevskiy [Australie] ; Nolan J. Hoffman [Australie] ; Naveid Ali [Australie] ; D. Neil Watkins [Australie] ; Roger J. Daly [Australie] ; David E. James [Australie] ; Thierry Lorca [France] ; Anna Castro [France] ; Andrew Burgess [Australie]Source :
- Molecular & Cellular Proteomics : MCP [ 1535-9476 ] ; 2015.
Descripteurs français
- KwdFr :
- Acides aminés (métabolisme), Anaphase, Cellules HeLa, Données de séquences moléculaires, Humains, Mitose, Modèles biologiques, Motifs d'acides aminés, Métaphase, Phosphopeptides (métabolisme), Phosphoprotéines (), Phosphoprotéines (métabolisme), Phosphorylation, Protein kinases (métabolisme), Protéomique (), Reproductibilité des résultats, Spécificité du substrat, Séquence conservée, Séquence d'acides aminés, Évolution moléculaire.
- MESH :
- métabolisme : Acides aminés, Phosphopeptides, Phosphoprotéines, Protein kinases.
- Anaphase, Cellules HeLa, Données de séquences moléculaires, Humains, Mitose, Modèles biologiques, Motifs d'acides aminés, Métaphase, Phosphoprotéines, Phosphorylation, Protéomique, Reproductibilité des résultats, Spécificité du substrat, Séquence conservée, Séquence d'acides aminés, Évolution moléculaire.
English descriptors
- KwdEn :
- Amino Acid Motifs, Amino Acid Sequence, Amino Acids (metabolism), Anaphase, Conserved Sequence, Evolution, Molecular, HeLa Cells, Humans, Metaphase, Mitosis, Models, Biological, Molecular Sequence Data, Phosphopeptides (metabolism), Phosphoproteins (chemistry), Phosphoproteins (metabolism), Phosphorylation, Protein Kinases (metabolism), Proteomics (methods), Reproducibility of Results, Substrate Specificity.
- MESH :
- chemical , chemistry : Phosphoproteins.
- chemical , metabolism : Amino Acids, Phosphopeptides, Phosphoproteins, Protein Kinases.
- methods : Proteomics.
- Amino Acid Motifs, Amino Acid Sequence, Anaphase, Conserved Sequence, Evolution, Molecular, HeLa Cells, Humans, Metaphase, Mitosis, Models, Biological, Molecular Sequence Data, Phosphorylation, Reproducibility of Results, Substrate Specificity.
Abstract
Entry into mitosis is driven by the coordinated phosphorylation of thousands of proteins. For the cell to complete mitosis and divide into two identical daughter cells it must regulate dephosphorylation of these proteins in a highly ordered, temporal manner. There is currently a lack of a complete understanding of the phosphorylation changes that occur during the initial stages of mitotic exit in human cells. Therefore, we performed a large unbiased, global analysis to map the very first dephosphorylation events that occur as cells exit mitosis. We identified and quantified the modification of >16,000 phosphosites on >3300 unique proteins during early mitotic exit, providing up to eightfold greater resolution than previous studies. The data have been deposited to the ProteomeXchange with identifier PXD001559. Only a small fraction (∼10%) of phosphorylation sites were dephosphorylated during early mitotic exit and these occurred on proteins involved in critical early exit events, including organization of the mitotic spindle, the spindle assembly checkpoint, and reformation of the nuclear envelope. Surprisingly this enrichment was observed across all kinase consensus motifs, indicating that it is independent of the upstream phosphorylating kinase. Therefore, dephosphorylation of these sites is likely determined by the specificity of phosphatase/s rather than the activity of kinase/s. Dephosphorylation was significantly affected by the amino acids at and surrounding the phosphorylation site, with several unique evolutionarily conserved amino acids correlating strongly with phosphorylation status. These data provide a potential mechanism for the specificity of phosphatases, and how they co-ordinate the ordered events of mitotic exit. In summary, our results provide a global overview of the phosphorylation changes that occur during the very first stages of mitotic exit, providing novel mechanistic insight into how phosphatase/s specifically regulate this critical transition.
Url:
DOI: 10.1074/mcp.M114.046938
PubMed: 26055452
PubMed Central: 4528247
Affiliations:
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Le document en format XML
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Neil Watkins</name><affiliation wicri:level="1"><nlm:aff id="aff1">From the ‡The Kinghorn Cancer Center, Garvan Institute of Medical Research, Darlinghurst, NSW, 2010, Australia;</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>From the ‡The Kinghorn Cancer Center, Garvan Institute of Medical Research, Darlinghurst, NSW, 2010</wicri:regionArea><wicri:noRegion>2010</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3">¶St. Vincent's Clinical School, Faculty of Medicine, UNSW, Darlinghurst, NSW, Australia;</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>¶St. Vincent's Clinical School, Faculty of Medicine, UNSW, Darlinghurst, NSW</wicri:regionArea><wicri:noRegion>NSW</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff4">‖Department of Thoracic Medicine, St Vincent's Hospital, Darlinghurst, NSW, 2010, Australia;</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>‖Department of Thoracic Medicine, St Vincent's Hospital, Darlinghurst, NSW, 2010</wicri:regionArea><wicri:noRegion>2010</wicri:noRegion></affiliation></author><author><name sortKey="Daly, Roger J" sort="Daly, Roger J" uniqKey="Daly R" first="Roger J." last="Daly">Roger J. 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France.</nlm:aff><country xml:lang="fr" wicri:curation="lc">France</country><wicri:regionArea>‡‡Equipe Labellisée Ligue Nationale Contre le Cancer, Universités Montpellier 2 et 1, Centre de Recherche de Biochimie Macromoléculaire, CNRS UMR 5237, 1919 Route de Mende, 34293 Montpellier cedex 5</wicri:regionArea><wicri:noRegion>34293 Montpellier cedex 5</wicri:noRegion><wicri:noRegion>34293 Montpellier cedex 5</wicri:noRegion></affiliation></author><author><name sortKey="Castro, Anna" sort="Castro, Anna" uniqKey="Castro A" first="Anna" last="Castro">Anna Castro</name><affiliation wicri:level="1"><nlm:aff id="aff6">‡‡Equipe Labellisée Ligue Nationale Contre le Cancer, Universités Montpellier 2 et 1, Centre de Recherche de Biochimie Macromoléculaire, CNRS UMR 5237, 1919 Route de Mende, 34293 Montpellier cedex 5, France.</nlm:aff><country xml:lang="fr" wicri:curation="lc">France</country><wicri:regionArea>‡‡Equipe Labellisée Ligue Nationale Contre le Cancer, Universités Montpellier 2 et 1, 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ref-type="fn" rid="FN1"><sup><inline-graphic xlink:href="sbox.jpg"></inline-graphic></sup></xref></title><author><name sortKey="Mccloy, Rachael A" sort="Mccloy, Rachael A" uniqKey="Mccloy R" first="Rachael A." last="Mccloy">Rachael A. Mccloy</name><affiliation wicri:level="1"><nlm:aff id="aff1">From the ‡The Kinghorn Cancer Center, Garvan Institute of Medical Research, Darlinghurst, NSW, 2010, Australia;</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>From the ‡The Kinghorn Cancer Center, Garvan Institute of Medical Research, Darlinghurst, NSW, 2010</wicri:regionArea><wicri:noRegion>2010</wicri:noRegion></affiliation></author><author><name sortKey="Parker, Benjamin L" sort="Parker, Benjamin L" uniqKey="Parker B" first="Benjamin L." last="Parker">Benjamin L. Parker</name><affiliation wicri:level="4"><nlm:aff id="aff2">§The Charles Perkins Center, School of Molecular Bioscience and Sydney Medical School, The University of Sydney, NSW 2006, Australia;</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>§The Charles Perkins Center, School of Molecular Bioscience and Sydney Medical School, The University of Sydney, NSW 2006</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Rogers, Samuel" sort="Rogers, Samuel" uniqKey="Rogers S" first="Samuel" last="Rogers">Samuel Rogers</name><affiliation wicri:level="1"><nlm:aff id="aff1">From the ‡The Kinghorn Cancer Center, Garvan Institute of Medical Research, Darlinghurst, NSW, 2010, Australia;</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>From the ‡The Kinghorn Cancer Center, Garvan Institute of Medical Research, Darlinghurst, NSW, 2010</wicri:regionArea><wicri:noRegion>2010</wicri:noRegion></affiliation></author><author><name sortKey="Chaudhuri, Rima" sort="Chaudhuri, Rima" uniqKey="Chaudhuri R" first="Rima" last="Chaudhuri">Rima Chaudhuri</name><affiliation wicri:level="4"><nlm:aff id="aff2">§The Charles Perkins Center, School of Molecular Bioscience and Sydney Medical School, The University of Sydney, NSW 2006, Australia;</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>§The Charles Perkins Center, School of Molecular Bioscience and Sydney Medical School, The University of Sydney, NSW 2006</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Gayevskiy, Velimir" sort="Gayevskiy, Velimir" uniqKey="Gayevskiy V" first="Velimir" last="Gayevskiy">Velimir Gayevskiy</name><affiliation wicri:level="1"><nlm:aff id="aff1">From the ‡The Kinghorn Cancer Center, Garvan Institute of Medical Research, Darlinghurst, NSW, 2010, Australia;</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>From the ‡The Kinghorn Cancer Center, Garvan Institute of Medical Research, Darlinghurst, NSW, 2010</wicri:regionArea><wicri:noRegion>2010</wicri:noRegion></affiliation></author><author><name sortKey="Hoffman, Nolan J" sort="Hoffman, Nolan J" uniqKey="Hoffman N" first="Nolan J." last="Hoffman">Nolan J. Hoffman</name><affiliation wicri:level="4"><nlm:aff id="aff2">§The Charles Perkins Center, School of Molecular Bioscience and Sydney Medical School, The University of Sydney, NSW 2006, Australia;</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>§The Charles Perkins Center, School of Molecular Bioscience and Sydney Medical School, The University of Sydney, NSW 2006</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Ali, Naveid" sort="Ali, Naveid" uniqKey="Ali N" first="Naveid" last="Ali">Naveid Ali</name><affiliation wicri:level="1"><nlm:aff id="aff1">From the ‡The Kinghorn Cancer Center, Garvan Institute of Medical Research, Darlinghurst, NSW, 2010, Australia;</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>From the ‡The Kinghorn Cancer Center, Garvan Institute of Medical Research, Darlinghurst, NSW, 2010</wicri:regionArea><wicri:noRegion>2010</wicri:noRegion></affiliation></author><author><name sortKey="Watkins, D Neil" sort="Watkins, D Neil" uniqKey="Watkins D" first="D. Neil" last="Watkins">D. Neil Watkins</name><affiliation wicri:level="1"><nlm:aff id="aff1">From the ‡The Kinghorn Cancer Center, Garvan Institute of Medical Research, Darlinghurst, NSW, 2010, Australia;</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>From the ‡The Kinghorn Cancer Center, Garvan Institute of Medical Research, Darlinghurst, NSW, 2010</wicri:regionArea><wicri:noRegion>2010</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3">¶St. Vincent's Clinical School, Faculty of Medicine, UNSW, Darlinghurst, NSW, Australia;</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>¶St. Vincent's Clinical School, Faculty of Medicine, UNSW, Darlinghurst, NSW</wicri:regionArea><wicri:noRegion>NSW</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff4">‖Department of Thoracic Medicine, St Vincent's Hospital, Darlinghurst, NSW, 2010, Australia;</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>‖Department of Thoracic Medicine, St Vincent's Hospital, Darlinghurst, NSW, 2010</wicri:regionArea><wicri:noRegion>2010</wicri:noRegion></affiliation></author><author><name sortKey="Daly, Roger J" sort="Daly, Roger J" uniqKey="Daly R" first="Roger J." last="Daly">Roger J. Daly</name><affiliation wicri:level="1"><nlm:aff id="aff5">**Department of Biochemistry and Molecular Biology, School of Biomedical Sciences Monash University, Clatyon, VIC, 3800, Australia;</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>**Department of Biochemistry and Molecular Biology, School of Biomedical Sciences Monash University, Clatyon, VIC, 3800</wicri:regionArea><wicri:noRegion>3800</wicri:noRegion></affiliation></author><author><name sortKey="James, David E" sort="James, David E" uniqKey="James D" first="David E." last="James">David E. James</name><affiliation wicri:level="4"><nlm:aff id="aff2">§The Charles Perkins Center, School of Molecular Bioscience and Sydney Medical School, The University of Sydney, NSW 2006, Australia;</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>§The Charles Perkins Center, School of Molecular Bioscience and Sydney Medical School, The University of Sydney, NSW 2006</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Lorca, Thierry" sort="Lorca, Thierry" uniqKey="Lorca T" first="Thierry" last="Lorca">Thierry Lorca</name><affiliation wicri:level="1"><nlm:aff id="aff6">‡‡Equipe Labellisée Ligue Nationale Contre le Cancer, Universités Montpellier 2 et 1, Centre de Recherche de Biochimie Macromoléculaire, CNRS UMR 5237, 1919 Route de Mende, 34293 Montpellier cedex 5, France.</nlm:aff><country xml:lang="fr" wicri:curation="lc">France</country><wicri:regionArea>‡‡Equipe Labellisée Ligue Nationale Contre le Cancer, Universités Montpellier 2 et 1, Centre de Recherche de Biochimie Macromoléculaire, CNRS UMR 5237, 1919 Route de Mende, 34293 Montpellier cedex 5</wicri:regionArea><wicri:noRegion>34293 Montpellier cedex 5</wicri:noRegion><wicri:noRegion>34293 Montpellier cedex 5</wicri:noRegion></affiliation></author><author><name sortKey="Castro, Anna" sort="Castro, Anna" uniqKey="Castro A" first="Anna" last="Castro">Anna Castro</name><affiliation wicri:level="1"><nlm:aff id="aff6">‡‡Equipe Labellisée Ligue Nationale Contre le Cancer, Universités Montpellier 2 et 1, Centre de Recherche de Biochimie Macromoléculaire, CNRS UMR 5237, 1919 Route de Mende, 34293 Montpellier cedex 5, France.</nlm:aff><country xml:lang="fr" wicri:curation="lc">France</country><wicri:regionArea>‡‡Equipe Labellisée Ligue Nationale Contre le Cancer, Universités Montpellier 2 et 1, Centre de Recherche de Biochimie Macromoléculaire, CNRS UMR 5237, 1919 Route de Mende, 34293 Montpellier cedex 5</wicri:regionArea><wicri:noRegion>34293 Montpellier cedex 5</wicri:noRegion><wicri:noRegion>34293 Montpellier cedex 5</wicri:noRegion></affiliation></author><author><name sortKey="Burgess, Andrew" sort="Burgess, Andrew" uniqKey="Burgess A" first="Andrew" last="Burgess">Andrew Burgess</name><affiliation wicri:level="1"><nlm:aff id="aff3">¶St. Vincent's Clinical School, Faculty of Medicine, UNSW, Darlinghurst, NSW, Australia;</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>¶St. Vincent's Clinical School, Faculty of Medicine, UNSW, Darlinghurst, NSW</wicri:regionArea><wicri:noRegion>NSW</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff1">From the ‡The Kinghorn Cancer Center, Garvan Institute of Medical Research, Darlinghurst, NSW, 2010, Australia;</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>From the ‡The Kinghorn Cancer Center, Garvan Institute of Medical Research, Darlinghurst, NSW, 2010</wicri:regionArea><wicri:noRegion>2010</wicri:noRegion></affiliation></author></analytic><series><title level="j">Molecular & Cellular Proteomics : MCP</title><idno type="ISSN">1535-9476</idno><idno type="eISSN">1535-9484</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Motifs</term><term>Amino Acid Sequence</term><term>Amino Acids (metabolism)</term><term>Anaphase</term><term>Conserved Sequence</term><term>Evolution, Molecular</term><term>HeLa Cells</term><term>Humans</term><term>Metaphase</term><term>Mitosis</term><term>Models, Biological</term><term>Molecular Sequence Data</term><term>Phosphopeptides (metabolism)</term><term>Phosphoproteins (chemistry)</term><term>Phosphoproteins (metabolism)</term><term>Phosphorylation</term><term>Protein Kinases (metabolism)</term><term>Proteomics (methods)</term><term>Reproducibility of Results</term><term>Substrate Specificity</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acides aminés (métabolisme)</term><term>Anaphase</term><term>Cellules HeLa</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Mitose</term><term>Modèles biologiques</term><term>Motifs d'acides aminés</term><term>Métaphase</term><term>Phosphopeptides (métabolisme)</term><term>Phosphoprotéines ()</term><term>Phosphoprotéines (métabolisme)</term><term>Phosphorylation</term><term>Protein kinases (métabolisme)</term><term>Protéomique ()</term><term>Reproductibilité des résultats</term><term>Spécificité du substrat</term><term>Séquence conservée</term><term>Séquence d'acides aminés</term><term>Évolution moléculaire</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Phosphoproteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Amino Acids</term><term>Phosphopeptides</term><term>Phosphoproteins</term><term>Protein Kinases</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Proteomics</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acides aminés</term><term>Phosphopeptides</term><term>Phosphoprotéines</term><term>Protein kinases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Motifs</term><term>Amino Acid Sequence</term><term>Anaphase</term><term>Conserved Sequence</term><term>Evolution, Molecular</term><term>HeLa Cells</term><term>Humans</term><term>Metaphase</term><term>Mitosis</term><term>Models, Biological</term><term>Molecular Sequence Data</term><term>Phosphorylation</term><term>Reproducibility of Results</term><term>Substrate Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Anaphase</term><term>Cellules HeLa</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Mitose</term><term>Modèles biologiques</term><term>Motifs d'acides aminés</term><term>Métaphase</term><term>Phosphoprotéines</term><term>Phosphorylation</term><term>Protéomique</term><term>Reproductibilité des résultats</term><term>Spécificité du substrat</term><term>Séquence conservée</term><term>Séquence d'acides aminés</term><term>Évolution moléculaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Entry into mitosis is driven by the coordinated phosphorylation of thousands of proteins. For the cell to complete mitosis and divide into two identical daughter cells it must regulate dephosphorylation of these proteins in a highly ordered, temporal manner. There is currently a lack of a complete understanding of the phosphorylation changes that occur during the initial stages of mitotic exit in human cells. Therefore, we performed a large unbiased, global analysis to map the very first dephosphorylation events that occur as cells exit mitosis. We identified and quantified the modification of >16,000 phosphosites on >3300 unique proteins during early mitotic exit, providing up to eightfold greater resolution than previous studies. The data have been deposited to the ProteomeXchange with identifier PXD001559. Only a small fraction (∼10%) of phosphorylation sites were dephosphorylated during early mitotic exit and these occurred on proteins involved in critical early exit events, including organization of the mitotic spindle, the spindle assembly checkpoint, and reformation of the nuclear envelope. Surprisingly this enrichment was observed across all kinase consensus motifs, indicating that it is independent of the upstream phosphorylating kinase. Therefore, dephosphorylation of these sites is likely determined by the specificity of phosphatase/s rather than the activity of kinase/s. Dephosphorylation was significantly affected by the amino acids at and surrounding the phosphorylation site, with several unique evolutionarily conserved amino acids correlating strongly with phosphorylation status. These data provide a potential mechanism for the specificity of phosphatases, and how they co-ordinate the ordered events of mitotic exit. In summary, our results provide a global overview of the phosphorylation changes that occur during the very first stages of mitotic exit, providing novel mechanistic insight into how phosphatase/s specifically regulate this critical transition.</p></div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li></country><region><li>Nouvelle-Galles du Sud</li></region><settlement><li>Sydney</li></settlement><orgName><li>Université de Sydney</li></orgName></list><tree><country name="Australie"><noRegion><name sortKey="Mccloy, Rachael A" sort="Mccloy, Rachael A" uniqKey="Mccloy R" first="Rachael A." last="Mccloy">Rachael A. Mccloy</name></noRegion><name sortKey="Ali, Naveid" sort="Ali, Naveid" uniqKey="Ali N" first="Naveid" last="Ali">Naveid Ali</name><name sortKey="Burgess, Andrew" sort="Burgess, Andrew" uniqKey="Burgess A" first="Andrew" last="Burgess">Andrew Burgess</name><name sortKey="Burgess, Andrew" sort="Burgess, Andrew" uniqKey="Burgess A" first="Andrew" last="Burgess">Andrew Burgess</name><name sortKey="Chaudhuri, Rima" sort="Chaudhuri, Rima" uniqKey="Chaudhuri R" first="Rima" last="Chaudhuri">Rima Chaudhuri</name><name sortKey="Daly, Roger J" sort="Daly, Roger J" uniqKey="Daly R" first="Roger J." last="Daly">Roger J. Daly</name><name sortKey="Gayevskiy, Velimir" sort="Gayevskiy, Velimir" uniqKey="Gayevskiy V" first="Velimir" last="Gayevskiy">Velimir Gayevskiy</name><name sortKey="Hoffman, Nolan J" sort="Hoffman, Nolan J" uniqKey="Hoffman N" first="Nolan J." last="Hoffman">Nolan J. Hoffman</name><name sortKey="James, David E" sort="James, David E" uniqKey="James D" first="David E." last="James">David E. James</name><name sortKey="Parker, Benjamin L" sort="Parker, Benjamin L" uniqKey="Parker B" first="Benjamin L." last="Parker">Benjamin L. Parker</name><name sortKey="Rogers, Samuel" sort="Rogers, Samuel" uniqKey="Rogers S" first="Samuel" last="Rogers">Samuel Rogers</name><name sortKey="Watkins, D Neil" sort="Watkins, D Neil" uniqKey="Watkins D" first="D. Neil" last="Watkins">D. Neil Watkins</name><name sortKey="Watkins, D Neil" sort="Watkins, D Neil" uniqKey="Watkins D" first="D. Neil" last="Watkins">D. Neil Watkins</name><name sortKey="Watkins, D Neil" sort="Watkins, D Neil" uniqKey="Watkins D" first="D. Neil" last="Watkins">D. Neil Watkins</name></country><country name="France"><noRegion><name sortKey="Lorca, Thierry" sort="Lorca, Thierry" uniqKey="Lorca T" first="Thierry" last="Lorca">Thierry Lorca</name></noRegion><name sortKey="Castro, Anna" sort="Castro, Anna" uniqKey="Castro A" first="Anna" last="Castro">Anna Castro</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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